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The Impact of cDNA Normalization on Long-Read Sequencing of a Complex Transcriptome

Normalization of cDNA is widely used to improve the coverage of rare transcripts in analysis of transcriptomes employing next-generation sequencing. Recently, long-read technology has been emerging as a powerful tool for sequencing and construction of transcriptomes, especially for complex genomes c...

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Dettagli Bibliografici
Pubblicato in:Front Genet
Autori principali: Hoang, Nam V., Furtado, Agnelo, Perlo, Virginie, Botha, Frederik C., Henry, Robert J.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Frontiers Media S.A. 2019
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6664245/
https://ncbi.nlm.nih.gov/pubmed/31396260
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fgene.2019.00654
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