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Analysis of N- and O-Glycosylation of Lysosomal Glycoproteins
The vast majority of lysosomal proteins are heavily glycosylated. The present protocol describes the method of analyzing N- and O-linked glycans in lysosomal proteins of interest. The method is based on using deglycosylating enzymes, endoglycosidases, and exoglycosidases. Endoglycosidases catalyze t...
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| Publicat a: | Methods Mol Biol |
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| Autors principals: | , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2017
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6663072/ https://ncbi.nlm.nih.gov/pubmed/28456975 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-6934-0_3 |
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