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Quantitative PCR primer design affects quantification of dsRNA‐mediated gene knockdown

RNA interference (RNAi) is a powerful tool for studying functions of candidate genes in both model and nonmodel organisms and a promising technique for therapeutic applications. Successful application of this technique relies on the accuracy and reliability of methods used to quantify gene knockdown...

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Detalhes bibliográficos
Publicado no:Ecol Evol
Main Authors: Onchuru, Thomas Ogao, Kaltenpoth, Martin
Formato: Artigo
Idioma:Inglês
Publicado em: John Wiley and Sons Inc. 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6662389/
https://ncbi.nlm.nih.gov/pubmed/31380081
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/ece3.5387
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