RNase I(f) -treated quantitative PCR for dsRNA quantitation of RNAi trait in genetically modified crops

BACKGROUND: RNA interference (RNAi) technology has been widely used to knockdown target genes via post-transcriptional silencing. In plants, RNAi is used as an effective tool with diverse applications being developed such as resistance against insects, fungi, viruses, and metabolism manipulation. To...

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Bibliographic Details
Published in:BMC Biotechnol
Main Authors: Wang, Po-Hao, Schulenberg, Greg, Whitlock, Shannon, Worden, Andrew, Zhou, Ning, Novak, Stephen, Chen, Wei
Format: Artigo
Language:Inglês
Published: BioMed Central 2018
Subjects:
Methodology Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5773123/
https://ncbi.nlm.nih.gov/pubmed/29343265
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12896-018-0413-6
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