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Proteome Analysis Using Gel-LC-MS/MS

This manuscript describes processing of protein samples using 1D SDS gels prior to protease digestion for proteomics workflows that subsequently utilize reversed-phase nanocapillary ultra-high pressure liquid chromatography (UPLC) coupled to tandem mass spectrometry (MS/MS). The resulting LC-MS/MS d...

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Détails bibliographiques
Publié dans:Curr Protoc Protein Sci
Auteurs principaux: Goldman, Aaron R., Beer, Lynn A., Tang, Hsin-Yao, Hembach, Peter, Zayas-Bazan, Delaine, Speicher, David W.
Format: Artigo
Langue:Inglês
Publié: 2019
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6653605/
https://ncbi.nlm.nih.gov/pubmed/31180188
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpps.93
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