Whole gel processing procedure for GeLC-MS/MS based proteomics

BACKGROUND: SDS-PAGE followed by in-gel digestion (IGD) is a popular workflow in mass spectrometry-based proteomics. In GeLC-MS/MS, a protein lysate of a biological sample is separated by SDS-PAGE and each gel lane is sliced in 5–20 slices which, after IGD, are analyzed by LC-MS/MS. The database sea...

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Bibliographic Details
Main Authors: Piersma, Sander R, Warmoes, Marc O, de Wit, Meike, de Reus, Inge, Knol, Jaco C, Jiménez, Connie R
Format: Artigo
Language:Inglês
Published: BioMed Central 2013
Subjects:
Methodology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3656797/
https://ncbi.nlm.nih.gov/pubmed/23617947
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1477-5956-11-17
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