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Whole gel processing procedure for GeLC-MS/MS based proteomics
BACKGROUND: SDS-PAGE followed by in-gel digestion (IGD) is a popular workflow in mass spectrometry-based proteomics. In GeLC-MS/MS, a protein lysate of a biological sample is separated by SDS-PAGE and each gel lane is sliced in 5–20 slices which, after IGD, are analyzed by LC-MS/MS. The database sea...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
BioMed Central
2013
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3656797/ https://ncbi.nlm.nih.gov/pubmed/23617947 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1477-5956-11-17 |
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