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Cryo-EM and directed evolution reveal how Arabidopsis nitrilase specificity is influenced by its quaternary structure

Nitrilases are helical enzymes that convert nitriles to acids and/or amides. All plants have a nitrilase 4 homolog specific for ß-cyanoalanine, while in some plants neofunctionalization has produced nitrilases with altered specificity. Plant nitrilase substrate size and specificity correlate with he...

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Detalhes bibliográficos
Publicado no:Commun Biol
Main Authors: Mulelu, Andani E., Kirykowicz, Angela M., Woodward, Jeremy D.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6637149/
https://ncbi.nlm.nih.gov/pubmed/31341959
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s42003-019-0505-4
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