TIE: A Method to Electroporate Long DNA Templates into Preimplantation Embryos for CRISPR-Cas9 Gene Editing

Precise genome editing using CRISPR typically requires delivery of guide RNAs, Cas9 endonuclease, and DNA repair templates. Both microinjection and electroporation effectively deliver these components into mouse zygotes provided the DNA template is an oligonucleotide of only a few hundred base pairs...

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Detalhes bibliográficos
Publicado no:CRISPR J
Main Authors: Bagheri, Hooman, Friedman, Hana, Shao, Harry, Chong, Yumaine, Lo, Chiu-An, Emran, Farida, Kays, Ibrahim, Yang, Xiang-Jiao, Cooper, Ellis, Chen, Brian E., Siminovitch, Katherine, Peterson, Alan
Formato: Artigo
Idioma:Inglês
Publicado em: Mary Ann Liebert, Inc. 2018
Assuntos:
Research Articles
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6636866/
https://ncbi.nlm.nih.gov/pubmed/31021258
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/crispr.2017.0020
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