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Microhomology-based CRISPR tagging tools for protein tracking, purification, and depletion
Work in yeast models has benefitted tremendously from the insertion of epitope or fluorescence tags at the native gene locus to study protein function and behavior under physiological conditions. In contrast, work in mammalian cells largely relies on overexpression of tagged proteins because high-qu...
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| Publicado no: | J Biol Chem |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Biochemistry and Molecular Biology
2019
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6635434/ https://ncbi.nlm.nih.gov/pubmed/31138654 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.RA119.008422 |
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