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SMURF-seq: efficient copy number profiling on long-read sequencers
We present SMURF-seq, a protocol to efficiently sequence short DNA molecules on a long-read sequencer by randomly ligating them to form long molecules. Applying SMURF-seq using the Oxford Nanopore MinION yields up to 30 fragments per read, providing an average of 6.2 and up to 7.5 million mappable f...
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| Udgivet i: | Genome Biol |
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| Main Authors: | , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
BioMed Central
2019
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6615205/ https://ncbi.nlm.nih.gov/pubmed/31287019 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-019-1732-1 |
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