A Strongly Fluorescing Anaerobic Reporter and Protein-Tagging System for Clostridium Organisms Based on the Fluorescence-Activating and Absorption-Shifting Tag Protein (FAST)

Visualizing protein localization and characterizing gene expression activity in live Clostridium cells is limited for lack of a real-time, highly fluorescent, oxygen-independent reporter system. Enzymatic reporter systems have been used successfully for many years with Clostridium spp.; however, the...

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Veröffentlicht in:Appl Environ Microbiol
Hauptverfasser: Streett, Hannah E., Kalis, Katie M., Papoutsakis, Eleftherios T.
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2019
Schlagworte:
Biotechnology
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6606882/
https://ncbi.nlm.nih.gov/pubmed/31076434
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00622-19
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