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MFEprimer-3.0: quality control for PCR primers

Quality control (QC) for lab-designed primers is crucial for the success of a polymerase chain reaction (PCR). Here, we present MFEprimer-3.0, a functional primer quality control program for checking non-specific amplicons, dimers, hairpins and other parameters. The new features of the current versi...

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Bibliografiske detaljer
Udgivet i:Nucleic Acids Res
Main Authors: Wang, Kun, Li, Haiwei, Xu, Yue, Shao, Qianzhi, Yi, Jianming, Wang, Ruichao, Cai, Wanshi, Hang, Xingyi, Zhang, Chenggang, Cai, Haoyang, Qu, Wubin
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2019
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Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6602485/
https://ncbi.nlm.nih.gov/pubmed/31066442
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkz351
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