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MFEprimer-2.0: a fast thermodynamics-based program for checking PCR primer specificity
Evaluating the specificity of polymerase chain reaction (PCR) primers is an essential step in PCR primer design. The MFEprimer-2.0 server allows users to check primer specificity against genomic DNA and messenger RNA/complementary DNA sequence databases quickly and easily. MFEprimer-2.0 uses a k-mer...
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| Main Authors: | , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3394324/ https://ncbi.nlm.nih.gov/pubmed/22689644 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gks552 |
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