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A thermodynamic approach to PCR primer design
We developed a primer design method, Pythia, in which state of the art DNA binding affinity computations are directly integrated into the primer design process. We use chemical reaction equilibrium analysis to integrate multiple binding energy calculations into a conservative measure of polymerase c...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Oxford University Press
2009
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2715258/ https://ncbi.nlm.nih.gov/pubmed/19528077 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp443 |
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