CRISPR-Cas9 fusion to dominant-negative 53BP1 enhances HDR and inhibits NHEJ specifically at Cas9 target sites

Precise genome editing/correction of DNA double-strand breaks (DSBs) induced by CRISPR-Cas9 by homology-dependent repair (HDR) is limited by the competing error-prone non-homologous end-joining (NHEJ) DNA repair pathway. Here, we define a safer and efficient system that promotes HDR-based precise ge...

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Vydáno v:Nat Commun
Hlavní autoři: Jayavaradhan, Rajeswari, Pillis, Devin M., Goodman, Michael, Zhang, Fan, Zhang, Yue, Andreassen, Paul R., Malik, Punam
Médium: Artigo
Jazyk:Inglês
Vydáno: Nature Publishing Group UK 2019
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6598984/
https://ncbi.nlm.nih.gov/pubmed/31253785
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-019-10735-7
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