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Development of a CRISPR/Cas9-Based Tool for Gene Deletion in Issatchenkia orientalis

The nonconventional yeast Issatchenkia orientalis has emerged as a potential platform microorganism for production of organic acids due to its ability to grow robustly under highly acidic conditions. However, lack of efficient genetic tools remains a major bottleneck in metabolic engineering of this...

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Veröffentlicht in:mSphere
Hauptverfasser: Tran, Vinh G., Cao, Mingfeng, Fatma, Zia, Song, Xiaofei, Zhao, Huimin
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2019
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6595149/
https://ncbi.nlm.nih.gov/pubmed/31243078
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/mSphere.00345-19
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