Comprehensive quantitation of RNA-protein interaction dynamics by orthogonal organic phase separation (OOPS)

Existing high-throughput methods to identify RNA-binding proteins (RBPs) involving capture of polyadenylated RNAs can not recover proteins that interact with non-adenylated RNAs, including lncRNA, pre-mRNA and bacterial RNAs. We present orthogonal organic phase separation (OOPS) which does not requi...

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Veröffentlicht in:Nat Biotechnol
Hauptverfasser: Queiroz, Rayner M. L., Smith, Tom, Villanueva, Eneko, Marti-Solano, Maria, Monti, Mie, Pizzinga, Mariavittoria, Mirea, Dan-Mircea, Ramakrishna, Manasa, Harvey, Robert F., Dezi, Veronica, Thomas, Gavin H., Willis, Anne E., Lilley, Kathryn S.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2019
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6591131/
https://ncbi.nlm.nih.gov/pubmed/30607034
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41587-018-0001-2
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