A fast and tuneable auxin‐inducible degron for depletion of target proteins in budding yeast

The auxin‐inducible degron (AID) is a useful technique to rapidly deplete proteins of interest in nonplant eukaryotes. Depletion is achieved by addition of the plant hormone auxin to the cell culture, which allows the auxin‐binding receptor, TIR1, to target the AID‐tagged protein for degradation by...

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Detalhes bibliográficos
Publicado no:Yeast
Main Authors: Mendoza‐Ochoa, Gonzalo I., Barrass, J. David, Terlouw, Barbara R., Maudlin, Isabella E., de Lucas, Susana, Sani, Emanuela, Aslanzadeh, Vahid, Reid, Jane A.E., Beggs, Jean D.
Formato: Artigo
Idioma:Inglês
Publicado em: John Wiley and Sons Inc. 2018
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Special Issue Articles
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6587778/
https://ncbi.nlm.nih.gov/pubmed/30375036
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/yea.3362
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