Impact of Host DNA and Sequencing Depth on the Taxonomic Resolution of Whole Metagenome Sequencing for Microbiome Analysis

The amount of host DNA poses a major challenge to metagenome analysis. However, there is no guidance on the levels of host DNA, nor on the depth of sequencing needed to acquire meaningful information from whole metagenome sequencing (WMS). Here, we evaluated the impact of a wide range of amounts of...

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Bibliografske podrobnosti
izdano v:Front Microbiol
Main Authors: Pereira-Marques, Joana, Hout, Anne, Ferreira, Rui M., Weber, Michiel, Pinto-Ribeiro, Ines, van Doorn, Leen-Jan, Knetsch, Cornelis Willem, Figueiredo, Ceu
Format: Artigo
Jezik:Inglês
Izdano: Frontiers Media S.A. 2019
Teme:
Microbiology
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC6581681/
https://ncbi.nlm.nih.gov/pubmed/31244801
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2019.01277
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