Impact of Host DNA and Sequencing Depth on the Taxonomic Resolution of Whole Metagenome Sequencing for Microbiome Analysis

The amount of host DNA poses a major challenge to metagenome analysis. However, there is no guidance on the levels of host DNA, nor on the depth of sequencing needed to acquire meaningful information from whole metagenome sequencing (WMS). Here, we evaluated the impact of a wide range of amounts of...

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Detalhes bibliográficos
Publicado no:Front Microbiol
Main Authors: Pereira-Marques, Joana, Hout, Anne, Ferreira, Rui M., Weber, Michiel, Pinto-Ribeiro, Ines, van Doorn, Leen-Jan, Knetsch, Cornelis Willem, Figueiredo, Ceu
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2019
Assuntos:
Microbiology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6581681/
https://ncbi.nlm.nih.gov/pubmed/31244801
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2019.01277
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