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Evolution of C-terminal modification tolerance in full-length and split T7 RNA Polymerase biosensors
T7 RNA polymerase (RNAP) is a powerful protein scaffold for the construction of synthetic biology tools and biosensors. However, both T7 RNAP and its split variants are intolerant to C-terminal modifications or fusions, placing a key limitation on its engineering and deployment. Here, we use rapid c...
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| Gepubliceerd in: | Chembiochem |
|---|---|
| Hoofdauteurs: | , , |
| Formaat: | Artigo |
| Taal: | Inglês |
| Gepubliceerd in: |
2019
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| Onderwerpen: | |
| Online toegang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6570552/ https://ncbi.nlm.nih.gov/pubmed/30694596 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.201800707 |
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