Evaluation of ultra-low input RNA sequencing for the study of human T cell transcriptome

Deeper understanding of T cell biology is crucial for the development of new therapeutics. Human naïve T cells have low RNA content and their numbers can be limiting; therefore we set out to determine the parameters for robust ultra-low input RNA sequencing. We performed transcriptome profiling at d...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Wang, Jingya, Rieder, Sadiye Amcaoglu, Wu, Jincheng, Hayes, Susana, Halpin, Rebecca A., de los Reyes, Melissa, Shrestha, Yashaswi, Kolbeck, Roland, Raja, Rajiv
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2019
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6559993/
https://ncbi.nlm.nih.gov/pubmed/31186477
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-44902-z
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