An improved Tet-on system in microRNA overexpression and CRISPR/Cas9-mediated gene editing

BACKGROUND: Tetracycline (Tet)-regulated expression system has become a widely applied tool to control gene activity. This study aimed to improve the Tet-on system with superior regulatory characteristics. RESULTS: By comprehensively comparing factors of transactivators, Tet-responsive elements (TRE...

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Veröffentlicht in:J Anim Sci Biotechnol
Hauptverfasser: Kang, Kang, Huang, Lian, Li, Qing, Liao, Xiaoyun, Dang, Quanjin, Yang, Yi, Luo, Jun, Zeng, Yan, Li, Li, Gou, Deming
Format: Artigo
Sprache:Inglês
Veröffentlicht: BioMed Central 2019
Schlagworte:
Research
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6556963/
https://ncbi.nlm.nih.gov/pubmed/31198556
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s40104-019-0354-5
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