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High-throughput identification of dominant negative polypeptides in yeast
Dominant negative polypeptides can inhibit protein function by binding to the wild type version or by titrating a ligand. Here, we use high-throughput sequencing of libraries composed of fragments of yeast genes to identify dominant negative polypeptides based on their depletion during cell growth....
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| Publicado no: | Nat Methods |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2019
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6555411/ https://ncbi.nlm.nih.gov/pubmed/30962621 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41592-019-0368-0 |
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