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Direct cloning and heterologous expression of natural product biosynthetic gene clusters by transformation-associated recombination

Heterologous expression of natural product biosynthetic gene clusters (BGCs) is a robust approach not only to decipher biosynthetic logic behind natural product (NP) biosynthesis, but also to discover new chemicals from uncharacterized BGCs. This approach largely relies on techniques used for clonin...

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Bibliographische Detailangaben
Veröffentlicht in:Methods Enzymol
Hauptverfasser: Zhang, Jia Jia, Yamanaka, Kazuya, Tang, Xiaoyu, Moore, Bradley S.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2019
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6555405/
https://ncbi.nlm.nih.gov/pubmed/31128791
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2019.02.026
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