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Direct cloning and heterologous expression of natural product biosynthetic gene clusters by transformation-associated recombination

Heterologous expression of natural product biosynthetic gene clusters (BGCs) is a robust approach not only to decipher biosynthetic logic behind natural product (NP) biosynthesis, but also to discover new chemicals from uncharacterized BGCs. This approach largely relies on techniques used for clonin...

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Detalhes bibliográficos
Publicado no:Methods Enzymol
Main Authors: Zhang, Jia Jia, Yamanaka, Kazuya, Tang, Xiaoyu, Moore, Bradley S.
Formato: Artigo
Idioma:Inglês
Publicado em: 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6555405/
https://ncbi.nlm.nih.gov/pubmed/31128791
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2019.02.026
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