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Direct cloning and heterologous expression of natural product biosynthetic gene clusters by transformation-associated recombination

Heterologous expression of natural product biosynthetic gene clusters (BGCs) is a robust approach not only to decipher biosynthetic logic behind natural product (NP) biosynthesis, but also to discover new chemicals from uncharacterized BGCs. This approach largely relies on techniques used for clonin...

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Publicado en:Methods Enzymol
Autores principales: Zhang, Jia Jia, Yamanaka, Kazuya, Tang, Xiaoyu, Moore, Bradley S.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2019
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC6555405/
https://ncbi.nlm.nih.gov/pubmed/31128791
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2019.02.026
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