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Global donor and acceptor splicing site kinetics in human cells

RNA splicing is an essential part of eukaryotic gene expression. Although the mechanism of splicing has been extensively studied in vitro, in vivo kinetics for the two-step splicing reaction remain poorly understood. Here, we combine transient transcriptome sequencing (TT-seq) and mathematical model...

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Veröffentlicht in:eLife
Hauptverfasser: Wachutka, Leonhard, Caizzi, Livia, Gagneur, Julien, Cramer, Patrick
Format: Artigo
Sprache:Inglês
Veröffentlicht: eLife Sciences Publications, Ltd 2019
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6548502/
https://ncbi.nlm.nih.gov/pubmed/31025937
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.45056
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