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Global donor and acceptor splicing site kinetics in human cells

RNA splicing is an essential part of eukaryotic gene expression. Although the mechanism of splicing has been extensively studied in vitro, in vivo kinetics for the two-step splicing reaction remain poorly understood. Here, we combine transient transcriptome sequencing (TT-seq) and mathematical model...

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Detalhes bibliográficos
Publicado no:eLife
Main Authors: Wachutka, Leonhard, Caizzi, Livia, Gagneur, Julien, Cramer, Patrick
Formato: Artigo
Idioma:Inglês
Publicado em: eLife Sciences Publications, Ltd 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6548502/
https://ncbi.nlm.nih.gov/pubmed/31025937
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.45056
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