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Marker-free genome editing in Ustilago trichophora with the CRISPR-Cas9 technology

In this communication, we report the adaptation of the CRISPR-Cas9 technology in Ustilago trichophora prototrophic wild-type isolate obtained from its natural host Echinochloa crus-galli. The established CRISPR vector and method enable a rapid and marker-free introduction of Cas9-induced non-homolog...

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Detalles Bibliográficos
Publicado en:RNA Biol
Main Authors: Huck, Simon, Bock, Josephine, Girardello, Jörg, Gauert, Marc, Pul, Ümit
Formato: Artigo
Idioma:Inglês
Publicado: Taylor & Francis 2018
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC6546355/
https://ncbi.nlm.nih.gov/pubmed/29996713
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/15476286.2018.1493329
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