Straightforward Protein-Protein Interaction Interface Mapping via Random Mutagenesis and Mammalian Protein Protein Interaction Trap (MAPPIT)

The MAPPIT (mammalian protein protein interaction trap) method allows high-throughput detection of protein interactions by very simple co-transfection of three plasmids in HEK293T cells, followed by a luciferase readout. MAPPIT detects a large percentage of all protein interactions, including those...

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Detalles Bibliográficos
Publicado en:Int J Mol Sci
Main Authors: Vyncke, Laurens, Masschaele, Delphine, Tavernier, Jan, Peelman, Frank
Formato: Artigo
Idioma:Inglês
Publicado: MDPI 2019
Assuntos:
Review
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC6539206/
https://ncbi.nlm.nih.gov/pubmed/31027327
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms20092058
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