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Oocyte Host-Transfer and Maternal mRNA Depletion Experiments in Xenopus
This protocol details the oocyte host-transfer method in Xenopus, using transplantation by intraperitoneal injection. This approach is suitable for the overexpression of mRNAs and for the use of antisense oligonucleotides to deplete maternal mRNAs, which are not replaced until zygotic genome activat...
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| Опубликовано в: : | Cold Spring Harb Protoc |
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| Главный автор: | |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
2018
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6538067/ https://ncbi.nlm.nih.gov/pubmed/29321286 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.prot096982 |
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