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Interdomain Fluorescence Resonance Energy Transfer in SERCA Probed by Cyan-Fluorescent Protein Fused to the Actuator Domain

We have used a biosynthetically incorporated fluorescent probe to monitor domain movements involved in ion transport by the sarcoendoplasmic reticulum Ca—ATPase (SERCA) from rabbit fast-twitch skeletal muscle. X-ray crystal structures suggest that the nucleotide-binding (N) and actuator (A) domains...

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Bibliografische gegevens
Gepubliceerd in:Biochemistry
Hoofdauteurs: Winters, Deborah L., Autry, Joseph M., Svensson, Bengt, Thomas, David D.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2008
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6537896/
https://ncbi.nlm.nih.gov/pubmed/18338856
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi702089j
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