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Interdomain Fluorescence Resonance Energy Transfer in SERCA Probed by Cyan-Fluorescent Protein Fused to the Actuator Domain
We have used a biosynthetically incorporated fluorescent probe to monitor domain movements involved in ion transport by the sarcoendoplasmic reticulum Ca—ATPase (SERCA) from rabbit fast-twitch skeletal muscle. X-ray crystal structures suggest that the nucleotide-binding (N) and actuator (A) domains...
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| Publicado no: | Biochemistry |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6537896/ https://ncbi.nlm.nih.gov/pubmed/18338856 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi702089j |
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