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A genetically encoded toolkit of functionalized nanobodies against fluorescent proteins for visualizing and manipulating intracellular signalling

BACKGROUND: Intrabodies enable targeting of proteins in live cells, but generating specific intrabodies against the thousands of proteins in a proteome poses a challenge. We leverage the widespread availability of fluorescently labelled proteins to visualize and manipulate intracellular signalling p...

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Detaylı Bibliyografya
Yayımlandı:BMC Biol
Asıl Yazarlar: Prole, David L., Taylor, Colin W.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: BioMed Central 2019
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC6533734/
https://ncbi.nlm.nih.gov/pubmed/31122229
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12915-019-0662-4
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