Generation of endogenous pH-sensitive EGF receptor and its application in high-throughput screening for proteins involved in clathrin-mediated endocytosis

Previously we used gene-editing to label endogenous EGF receptor (EGFR) with GFP and demonstrate that picomolar concentrations of EGFR ligand drive signaling and endocytosis of EGFR in tumors in vivo (Pinilla-Macua et al., 2017). We now use gene-editing to insert a fluorogen activating protein (FAP)...

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Veröffentlicht in:eLife
Hauptverfasser: Larsen, Mads Breum, Perez Verdaguer, Mireia, Schmidt, Brigitte F, Bruchez, Marcel P, Watkins, Simon C, Sorkin, Alexander
Format: Artigo
Sprache:Inglês
Veröffentlicht: eLife Sciences Publications, Ltd 2019
Schlagworte:
Cell Biology
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6533059/
https://ncbi.nlm.nih.gov/pubmed/31066673
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.46135
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