MS2 Labeling of Endogenous Beta-Actin mRNA Does Not Result in Stabilization of Degradation Intermediates

The binding of MS2 bacteriophage coat protein (MCP) to MS2 binding site (MBS) RNA stem-loop sequences has been widely used to label mRNA for live-cell imaging at single-molecule resolution. However, concerns have been raised recently from studies with budding yeast showing aberrant mRNA metabolism f...

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Bibliographische Detailangaben
Veröffentlicht in:Mol Cells
Hauptverfasser: Kim, Songhee H., Vieira, Melissa, Kim, Hye-Jin, Kesawat, Mahipal Singh, Park, Hye Yoon
Format: Artigo
Sprache:Inglês
Veröffentlicht: Korean Society for Molecular and Cellular Biology 2019
Schlagworte:
Rapid Report
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6530646/
https://ncbi.nlm.nih.gov/pubmed/30841028
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.14348/molcells.2019.2398
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