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A transgenic mouse for in vivo detection of endogenous labeled mRNA

Live-cell single mRNA imaging is a powerful tool, but has been restricted in higher eukaryotes to artificial cell lines and reporter genes. We describe an approach that enables live-cell imaging of single endogenous labeled mRNA molecules transcribed in primary mammalian cells and tissue. We generat...

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Detalhes bibliográficos
Main Authors: Lionnet, Timothée, Czaplinski, Kevin, Darzacq, Xavier, Shav-Tal, Yaron, Wells, Amber L., Chao, Jeffrey A., Park, Hye Yoon, de Turris, Valeria, Lopez-Jones, Melissa, Singer, Robert H.
Formato: Artigo
Idioma:Inglês
Publicado em: 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3076588/
https://ncbi.nlm.nih.gov/pubmed/21240280
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1551
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