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Non-canonical amino acid labeling in proteomics and biotechnology

Metabolic labeling of proteins with non-canonical amino acids (ncAAs) provides unique bioorthogonal chemical groups during de novo synthesis by taking advantage of both endogenous and heterologous protein synthesis machineries. Labeled proteins can then be selectively conjugated to fluorophores, aff...

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Détails bibliographiques
Publié dans:J Biol Eng
Auteurs principaux: Saleh, Aya M., Wilding, Kristen M., Calve, Sarah, Bundy, Bradley C., Kinzer-Ursem, Tamara L.
Format: Artigo
Langue:Inglês
Publié: BioMed Central 2019
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6529998/
https://ncbi.nlm.nih.gov/pubmed/31139251
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13036-019-0166-3
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