A Two-Step Method for Obtaining Highly Pure Cas9 Nuclease for Genome Editing, Biophysical, and Structural Studies

Cas9 is a site-specific RNA-guided endonuclease (RGEN) that can be used for precise genome editing in various cell types from multiple species. Ribonucleoprotein (RNP) complexes, which contains the Cas9 protein in complex with a guide RNA, are sufficient for the precise editing of genomes in various...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Methods Protoc
Main Authors: Rajagopalan, Nandhakishore, Kagale, Sateesh, Bhowmik, Pankaj, Song, Halim
Format: Artigo
Jezik:Inglês
Izdano: MDPI 2018
Teme:
Protocol
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC6526429/
https://ncbi.nlm.nih.gov/pubmed/31164560
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/mps1020017
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