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Reduced False Positives and Improved Reporting of Loop-Mediated Isothermal Amplification using Quenched Fluorescent Primers

Loop-mediated isothermal amplification (LAMP) is increasingly used in molecular diagnostics as an alternative to PCR based methods. There are numerous reported techniques to detect the LAMP amplification including turbidity, bioluminescence and intercalating fluorescent dyes. In this report we show...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Hardinge, Patrick, Murray, James A. H.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6517417/
https://ncbi.nlm.nih.gov/pubmed/31089184
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-43817-z
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