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Reduced False Positives and Improved Reporting of Loop-Mediated Isothermal Amplification using Quenched Fluorescent Primers

Loop-mediated isothermal amplification (LAMP) is increasingly used in molecular diagnostics as an alternative to PCR based methods. There are numerous reported techniques to detect the LAMP amplification including turbidity, bioluminescence and intercalating fluorescent dyes. In this report we show...

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Vydáno v:Sci Rep
Hlavní autoři: Hardinge, Patrick, Murray, James A. H.
Médium: Artigo
Jazyk:Inglês
Vydáno: Nature Publishing Group UK 2019
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6517417/
https://ncbi.nlm.nih.gov/pubmed/31089184
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-43817-z
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