Capture and display of antibodies secreted by hybridoma cells enables fluorescent on-cell screening

Hybridoma methods for monoclonal antibody (mAb) cloning are a mainstay of biomedical research, but they are hindered by the need to maintain hybridomas in oligoclonal pools during antibody screening. Here, we describe a system in which hybridomas specifically capture and display the mAbs they secret...

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Detalhes bibliográficos
Publicado no:MAbs
Main Authors: Puligedda, Rama Devudu, Sharma, Rashmi, Al-Saleem, Fetweh H., Kouiavskaia, Diana, Velu, Arul Balaji, Kattala, Chandana Devi, Prendergast, George C., Lynch, David R., Chumakov, Konstantin, Dessain, Scott K.
Formato: Artigo
Idioma:Inglês
Publicado em: Taylor & Francis 2019
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6512912/
https://ncbi.nlm.nih.gov/pubmed/30794061
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/19420862.2019.1574520
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