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Combining patch-clamping and fluorescence microscopy for quantitative reconstitution of cellular membrane processes with Giant Suspended Bilayers

In vitro reconstitution and microscopic visualization of membrane processes is an indispensable source of information about a cellular function. Here we describe a conceptionally novel free-standing membrane template that facilitates such quantitative reconstitution of membrane remodelling at differ...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Velasco-Olmo, Ariana, Ormaetxea Gisasola, Julene, Martinez Galvez, Juan Manuel, Vera Lillo, Javier, Shnyrova, Anna V.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6510758/
https://ncbi.nlm.nih.gov/pubmed/31076583
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-43561-4
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