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Generating Pancreatic Duct Organoids and Whole- Mount Immunostaining of Intact Organoids

Traditionally, studies on cells and tissues have been performed on isolated primary cells or immortalized cell lines by culturing them in 2D culture dishes or flasks. However, a caveat of 2D culture is that the cells poorly recapitulate their in vivo counterparts mainly due to the lack of 3D cell-ce...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Curr Protoc Cell Biol
Main Authors: Rezanejad, Habib, Lock, Jennifer Hollister, Sullivan, Brooke A, Bonner-Weir, Susan
Format: Artigo
Jezik:Inglês
Izdano: 2018
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC6506379/
https://ncbi.nlm.nih.gov/pubmed/30548444
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpcb.82
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