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Generating Pancreatic Duct Organoids and Whole- Mount Immunostaining of Intact Organoids
Traditionally, studies on cells and tissues have been performed on isolated primary cells or immortalized cell lines by culturing them in 2D culture dishes or flasks. However, a caveat of 2D culture is that the cells poorly recapitulate their in vivo counterparts mainly due to the lack of 3D cell-ce...
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| Published in: | Curr Protoc Cell Biol |
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| Main Authors: | , , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
2018
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6506379/ https://ncbi.nlm.nih.gov/pubmed/30548444 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpcb.82 |
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