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Gαi3 binding to calnuc on Golgi membranes in living cells monitored by fluorescence resonance energy transfer of green fluorescent protein fusion proteins

Gαi3 is found both on the plasma membrane and on Golgi membranes. Calnuc, an EF hand protein, binds both Gαi3 and Ca(2+) and is found both in the Golgi lumen and in the cytoplasm. To investigate whether Gαi3 binds calnuc in living cells and where this interaction takes place we performed fluorescenc...

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Detalhes bibliográficos
Main Authors: Weiss, Thomas S., Chamberlain, Chester E., Takeda, Tetsuro, Lin, Ping, Hahn, Klaus M., Farquhar, Marilyn Gist
Formato: Artigo
Idioma:Inglês
Publicado em: The National Academy of Sciences 2001
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC64966/
https://ncbi.nlm.nih.gov/pubmed/11752444
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.261572098
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