Gαi3 binding to calnuc on Golgi membranes in living cells monitored by fluorescence resonance energy transfer of green fluorescent protein fusion proteins

Gαi3 is found both on the plasma membrane and on Golgi membranes. Calnuc, an EF hand protein, binds both Gαi3 and Ca(2+) and is found both in the Golgi lumen and in the cytoplasm. To investigate whether Gαi3 binds calnuc in living cells and where this interaction takes place we performed fluorescenc...

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Bibliographic Details
Main Authors: Weiss, Thomas S., Chamberlain, Chester E., Takeda, Tetsuro, Lin, Ping, Hahn, Klaus M., Farquhar, Marilyn Gist
Format: Artigo
Language:Inglês
Published: The National Academy of Sciences 2001
Subjects:
Biological Sciences
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC64966/
https://ncbi.nlm.nih.gov/pubmed/11752444
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.261572098
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