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Generation, expression and utilization of single-domain antibodies for in vivo protein localization and manipulation in sea urchin embryos

Single-domain antibodies, also known as nanobodies, are small antigen-binding fragments (~15 kDa) that are derived from heavy chain only antibodies present in camelids (V(HH), from camels and llamas), and cartilaginous fishes (V(NAR), from sharks). Nanobody V-like domains are useful alternatives to...

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Bibliografski detalji
Izdano u:Methods Cell Biol
Glavni autori: Schrankel, Catherine S., Gökirmak, Tufan, Lee, Chang-Wook, Chang, Geoffrey, Hamdoun, Amro
Format: Artigo
Jezik:Inglês
Izdano: 2019
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6489465/
https://ncbi.nlm.nih.gov/pubmed/30948018
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mcb.2018.11.005
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