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CRISPR directed evolution of the spliceosome for resistance to splicing inhibitors

Increasing genetic diversity via directed evolution holds great promise to accelerate trait development and crop improvement. We developed a CRISPR/Cas-based directed evolution platform in plants to evolve the rice (Oryza sativa) SF3B1 spliceosomal protein for resistance to splicing inhibitors. SF3B...

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Detalhes bibliográficos
Publicado no:Genome Biol
Main Authors: Butt, Haroon, Eid, Ayman, Momin, Afaque A., Bazin, Jeremie, Crespi, Martin, Arold, Stefan T., Mahfouz, Magdy M.
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6489355/
https://ncbi.nlm.nih.gov/pubmed/31036069
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-019-1680-9
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