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Nontoxic nanopore electroporation for effective intracellular delivery of biological macromolecules

We present a simple nanopore-electroporation (NanoEP) platform for delivery of nucleic acids, functional protein, and Cas9 single-guide RNA ribonucleoproteins into both adherent and suspension cells with up to 80% delivery efficiency and >95% cell viability. Low-voltage electric pulses permeabili...

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Bibliografische gegevens
Gepubliceerd in:Proc Natl Acad Sci U S A
Hoofdauteurs: Cao, Yuhong, Ma, Enbo, Cestellos-Blanco, Stefano, Zhang, Bei, Qiu, Ruoyi, Su, Yude, Doudna, Jennifer A., Yang, Peidong
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: National Academy of Sciences 2019
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6475394/
https://ncbi.nlm.nih.gov/pubmed/30923112
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1818553116
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