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Nontoxic nanopore electroporation for effective intracellular delivery of biological macromolecules
We present a simple nanopore-electroporation (NanoEP) platform for delivery of nucleic acids, functional protein, and Cas9 single-guide RNA ribonucleoproteins into both adherent and suspension cells with up to 80% delivery efficiency and >95% cell viability. Low-voltage electric pulses permeabili...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Main Authors: | , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2019
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6475394/ https://ncbi.nlm.nih.gov/pubmed/30923112 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1818553116 |
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